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. 2017 Oct 26;7:14136. doi: 10.1038/s41598-017-14095-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of Cameroon HIV-1 pol sequences. Pol nucleotide sequences of 125 clinical HIV-1 isolates from Cameroon (NACMR or NA2CMR IDs) were aligned using ClustalW, and phylogenetic analysis performed using the neighbor-joining method of MEGA.v.5 software as described in the Methods section. The reference sequences were from the Los Alamos database and included HIV-1 isolates from twelve countries (country letters code precedes reference accession number). Some references have been omitted to enable better visualization of the new Cameroon HIV sequences: CRF02_AG (pink) and non-CRF02_AG (green) subtypes. The Bootstrap value of 1000 replicates of at least 70% was used to determine the HIV-1 subtype. The scale bar represents 2% genetic distance.