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. 2017 Oct 26;7:14143. doi: 10.1038/s41598-017-14396-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of neonatal hyperoxia in ischemic tissues. Capillary density (A,B) and arteriolar density (C) in ischemic muscles harvested at day 21 after surgery in the different groups of mice. Arrows in (A) indicate positive (brown) CD31 staining in capillaries. Isch = ischemic. N-Isch = non ischemic. (D) Representative Western blots and quantitative analyses of VEGF expression in ischemic muscles at day 7 after surgery. Data were normalized using loading controls (alpha-tubulin) and are presented as mean ± SEM (n = 4/ group). (E) Effect of hyperoxia exposure on the level of angiogenic factors in the serum, as evaluated by Elisa at day 7 after surgery. *P < 0.05 vs. normoxia.