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. 2017 Oct 26;7:14077. doi: 10.1038/s41598-017-13688-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cyclin reporter translation dependence on CPEB1. (A) CPEB binding to Ccnb1, Ccnb2, and Mos mRNAs. After immunoprecipitation of GV oocyte lysate with CPEB1 specific antibody, transcripts recovered in the pellet were reverse-transcribed followed by quantitative PCR with specific primers. The graphs report the mean ± SEM of three independent experiments. (B–D) CPEB1 was depleted from the oocytes by injection of morpholino oligomer (MO) or control MO. Either the Ccnb1 or Ccnb2 3′UTR reporter was injected at the same time. After a 24 h incubation, milrinone was removed to induce cell cycle reentry, and the translation of Ccnb1 (C) or Ccnb2 reporter (D) was measured at 6 or 8 h after resumption of meiosis. The graphs are the mean ± SEM of three independent experiments.