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. 2017 Oct 26;7:14161. doi: 10.1038/s41598-017-14036-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of experimental set up and 20 m of path from all ants tested. (A) Ants travelled a route between a feeder (F) 8 m from their nest (N) and their home. Experienced ants were taken for a test after 2 days of shuttling back and forth, while naïve ants were taken after their first arrival. Ants were taken either from the feeder (8 m condition, 8 m of vector remaining), half way on the route home (4 m condition, 4 m of vector remaining) or just before they entered their nest (0 m condition, 0 m of vector remaining). PI = path integration. (B) All paths run by the tested ants divided into the first 10 m of path length and the second 10 m of path length. A circle 2 m in radius has been drawn around each graph to help readers visualise the starting region on tests. No such circle was actually drawn at the test site.