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. 2017 Feb 14;10(6):1535–1545. doi: 10.1111/1751-7915.12486

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© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Ethanol production by AdhE‐containing strains of Pyrococcus furiosus.

All strains, aside from COM1c2, have AOR deleted. P. furiosus strains and two control strains (ΔAORc and COM1c2) were grown in closed serum bottles with 50 ml YM5 medium to mid‐log phase at 95°C and then incubated at 65°C for 90 h.

A. Ethanol production over time.

B. Ethanol produced per estimated glucose consumed, calculated from ethanol and acetate values for the 90 h time point (shown in A), ordered from highest to lowest. Ethanol and acetate are assumed to be the only carbon end‐products after glycolysis. Error bars represent SD, n = 3.