Table 2.

Pyrococcus furiosus strains used and constructed in this study

Strain	Alias	Genotypea	Reference
Genetic background and control strains
MW002	COM1	ΔpyrF	Lipscomb et al. (2011)
MW252	ΔAOR	ΔpyrF Δaor	This work
MW004	COM1c2	ΔpyrF::pyrF	Thorgersen et al. (2012)
MW616	ΔAORc	ΔpyrF Δaor::Pgdh pyrF	This work
MW611	AdhA	ΔpyrF GR3::PslpTxadhA Δaor::Pgdh pyrF	Basen et al. (2014)
AdhE expression strains
MW627	Ct‐AdhE	ΔpyrF Δaor GR4::Pslp CtadhE Pgdh pyrF	This work
MW332	Tx‐AdhE	ΔpyrF Δaor GR4::Pslp TxadhE Pgdh pyrF	This work
MW371	Gt‐AdhE	ΔpyrF Δaor GR4::Pslp GtadhE Pgdh pyrF	This work
MW364	Dk‐AdhE	ΔpyrF Δaor GR4::Pslp DkadhE Pgdh pyrF	This work
MW373	Af‐AdhE	ΔpyrF Δaor GR4::Pslp AfadhE Pgdh pyrF	This work
MW357	Gs‐AdhE	ΔpyrF Δaor GR4::Pslp GsadhE Pgdh pyrF	This work
MW372	Tc‐AdhE	ΔpyrF Δaor GR4::Pslp TcadhE Pgdh pyrF	This work
MW392	Te‐AdhE	ΔpyrF Δaor GR4::Pslp TeadhE Pgdh pyrF	This work
MW325	Te‐AdhEA	ΔpyrF Δaor GR4::Pslp TeadhE TxadhA Pgdh pyrF	This work
Mutant AdhE expression strains
MW623	Tx‐AdhE*	ΔpyrF Δaor GR4::Pslp TxadhE(G557D) Pgdh pyrF	This work
MW625	Te‐AdhE*	ΔpyrF Δaor GR4::Pslp TeadhE(G532D) Pgdh pyrF	This work

^aGR3 represents insertion at genome region 3 (between genes PF0574 and PF0575), and GR4 represents insertion at genome region 4 (between genes PF PF0738.1n and PF0739). Organism abbreviations are listed in Table 1.