Table 1.
Microbial polyester hydrolases reported to cause significant weight losses of PET materials
| Enzyme | Source | Reaction conditions | Weight loss | Crystallinity of PET | PET source and preparation | References | |
|---|---|---|---|---|---|---|---|
| Temperature | Time | ||||||
| TfH | Thermobifida fusca DSM43793 | 55°C | 3 weeks | ≈ 50% | 10% | Melt pressing of a beverage bottle (Granini AG, Nieder‐Olm, Germany) | (Müller et al., 2005) |
| TfH | Thermobifida fusca DSM43793 | 55‐65°C | 48 h | ≤ 14% | Not reported | Amorphous PET film (Goodfellow GmbH, Bad Nauheim, Germany) | (Then et al., 2015) |
| BTA‐2 | ≤ 4% | ||||||
| Tfu_0882 | Thermobifida fusca YX | 55‐65°C | 48 h | ≤ 5% | (Then et al., 2015) | ||
| TfCut1 | Thermobifida fusca KW3 | 55‐65°C | 48 h | ≤ 11% | (Then et al., 2015) | ||
| TfCut2 | ≤ 12% | ||||||
| Variants of TfCut2 | Thermobifida fusca KW3 | 65‐80°C | 48 h | ≤ 25% | (Then et al., 2015, 2016) | ||
| 65°C | 50 h | ≤ 45% | (Wei et al., 2016) | ||||
| A double mutant of Cut190 | Saccharomonospora viridis AHK190 | 63°C | 3 days | 13.5 ± 0.5% | Not reported | Amorphous PET film (Goodfellow Cambridge, Ltd., Tokyo, Japan) | (Kawai et al., 2014) |
| 27 ± 1% | Amorphous PET film (Sanwa Supply Inc., Okayama, Japan) | ||||||
| LC‐cutinase | Metagenome from plant compost | 50‐70°C | 24 h | ≤ 25% | Not reported | Amorphous PET film (Sanwa Supply Inc., Okayama, Japan) | (Sulaiman et al., 2014) |
| HiC | Thermomyces (formerly Humicola) insolens | 70°C | 96 h | 97 ± 3% | 7% | Amorphous PET film (Goodfellow Cambridge, Ltd.) | (Ronkvist et al., 2009) |