Table 1.
Extracellular Carbohydrate Active Enzymes from Caldicellulosiruptor saccharolyticus
| Fold changea | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Locus tag | Annotation/Activity | SigP | TM | AA | CAZy Family | Cellulose versus | Xylan versus | ||||
| KGM | P | X | CC | KGM | P | ||||||
| Csac_0408 | α‐Amylase | Y | N | 514 | GH13 | – | – | – | 4.3 | – | – |
| Csac_0678 | Endoglucanase/endo‐1,4‐β‐xylanase | Y | N | 756 | GH5, CBM28 | 12.8 | 11.4 | 8.8 | – | – | – |
| Csac_0689 | Pullulanase | Y | N | 1136 | CBM41, CBM48,GH13, CBM20 | – | – | – | 10.7 | 2.4 | 4.4 |
| Csac_0696 | Endo‐1,4‐β‐xylanase | Y | N | 686 | 2× CBM22, GH10 | – | – | – | 148.6 | 35.7 | 31.3 |
| Csac_0719 | Acetyl xylan esterase | N | Y | 299 | CE4 | – | – | – | – | – | – |
| Csac_1076 | Endo‐1,4‐β‐glucanase (CelA) | Y | N | 1751 | GH9, 3× CBM3, GH48 | 2.2 | 7.4 | 3.3 | – | – | 2.2 |
| Csac_1077 | Endo‐1,4‐β‐glucanase/β‐1,4‐mannanase; endo‐1,4‐β‐xylanase (ManA) | Y | N | 1303 | GH5, 2× CBM3, GH44 | 60.9 | 89.3 | 35.1 | – | – | 2.6 |
| Csac_1078 | Endo‐1,4‐β‐xylanase/endo‐1,4‐β‐glucanase; β‐1,4‐glucomannanase (CelB) | Y | N | 1039 | GH10, CBM3, GH5 | – | 3.3 | – | – | – | 2.7 |
| Csac_1079 | Endo‐1,4‐β‐glucanase (CelC) | Y | N | 1127 | GH9, 3× CBM3 | 8.6 | 8.4 | 4.8 | – | – | – |
| Csac_1081 | Protein α‐mannosyltransferase | N | Y | 565 | GT39 | – | 2.9 | – | – | – | – |
| Csac_1085 | Endoglucanase/xyloglucanase | Y | N | 931 | GH74, CBM3 | – | – | – | 2.8 | – | – |
| Csac_1560 | Endo‐1,5‐α‐L‐arabinase | Y | N | 488 | GH43 | – | – | – | 20.8 | 2.7 | 5.8 |
| Csac_2009 | Acetyl xylan esterase | Y | N | 258 | CE4 | – | – | – | – | – | – |
| Csac_2410 | Endo‐1,4‐β‐xylanase (XynE) | Y | N | 700 | 2 × CBM22, GH10 | 5.6 | 4.3 | 2.3 | |||
| Csac_2411 | α‐L‐Arabinofuranosidase (XynF) | Y | N | 1347 | GH43, CBM22, GH43, CBM6 | 2.3 | 2.5 | 1.9 | – | – | – |
| Csac_2371 | Acetyl xylan esterase | Y | N | 322 | CE4 | – | – | – | – | – | – |
| Csac_2519 | Carbohydrate binding protein | Y | N | 628 | CBM32 | – | – | – | – | – | – |
| Csac_2528 | Endoglucanase | Y | N | 611 | GH5 | – | – | – | 2.8 | – | – |
| Csac_2722 | Hypothetical protein/carbohydrate binding | Y | N | 2593 | GHnc | – | – | – | 3.8 | – | – |
SigP, signal peptide; TM, transmembrane domain; AA, amino acid length; CC, crystalline cellulose; KGM, konjac glucomannan; P, pectin; X, xylan.
a. Fold change values are calculated from log2 ratios of crystalline cellulose or xylan versus other conditions listed in the columns below. Grey shading indicates statistical significance.