Table 1.
The percentage of CC and CXC chemokines removed from solution after incubation with 2 × 109 or 1 × 1010 L. lactis NZ9000 cells that displayed on their surface evasin‐1 (pSDEva1), evasin‐4 (pSDEva4) or evasin‐3 (pSDEva3), relative to empty plasmid‐containing control cells. Chemokine concentration was determined with ELISA and multiplexing Luminex assay system MAGPIX
| L. lactis NZ9000 | Determination methods | Number of cells | ||
|---|---|---|---|---|
| pSDEva1 | pSDEva4 | |||
| CCL3 (MIP‐1α) | 23.2 ± 5.1 | n.b. | ELISA | 2 × 109 |
| CCL4 (MIP‐1β) | n.b | n.b. | ||
| CCL5 (RANTES) | n.b | 38.7 ± 9.6 | ||
| CCL3 (MIP‐1α) | 65.8 | 48.5 | LUMINEX | 1 × 1010 |
| CCL11 (Eotaxin) | n.b. | 23.2 | ||
| CCL18 (PARC) | n.b. | 19.5 | ||
| CCL24 (Eotaxin‐2) | n.b. | n.b. | ||
| CCL25 (TECK) | n.b. | 29.3 | ||
| pSDEva3 | ||||
| mCXCL1 (KC) | 56.7 ± 7.0 | ELISA | 2 × 109 | |
| CXCL2 (Gro‐β) | 44.1 ± 1.5 | |||
| mCXCL2 (MIP‐2) | 67.0 ± 6.6 | |||
| CXCL8 (IL‐8) | 54.5 ± 2.1 | |||
| CXCL1 (Gro‐α) | n.b. | LUMINEX | 2 × 109 | |
| CXCL4 (PF4) | n.b. | |||
| CXCL5 (ENA78) | 16.7 ± 1.4 | |||
| CXCL6 (GCP‐2) | 20.0 ± 4.0 | |||
| CXCL16 | n.b. | |||
n.b., no statistically significant binding. Murine chemokines are denoted by m.