Figure 4.

Growth and benzoate consumption of Azoarcus sp. CIB harbouring plasmid pIZ1016 (circles) or the pLB1 plasmid (triangles). Bacteria were cultivated anaerobically at 30°C in minimal MC medium containing 3 mM benzoate and 10 mM nitrate. Bacterial growth, monitored by measuring the absorbance at 600 nm (A ₆₀₀), is indicated with a continuous line. The concentration of benzoate in the culture medium was monitored spectrophotometrically at 273 nm, and the percentage of benzoate remaining in the culture medium is indicated with a dashed line. Values are the mean of three different experiments. Error bars indicate standard deviation.