Table 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Relevant genotype and main characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | endA1 hsdR17 supE44 this‐1 recA1 gyrA(Nar r ) relA1 Δ/argF‐lac) U169 depRФ80dlacd(lacZ)M15 | Sambrook and Russell, (2001) |
| S17‐1λpir | Tpr Smr recA thi hsdRM + RP4׃׃2.Tc׃׃Mu׃׃Km Tn7 λpir phage lysogen | de Lorenzo and Timmis, (1994) |
| Azoarcus strains | ||
| Azoarcus sp. CIB | Wild‐type strain | López‐Barragán et al., (2004) |
| Azoarcus sp. CIBdbzdN | Kmr , Azoarcus sp. CIB with a disruption in the bzdN gene | López‐Barragán et al., (2004) |
| Azoarcus communis SWub3 | Wild‐ type strain (LMG22127) | Reinhold‐Hurek et al., (1993) |
| Azoarcus sp. CIB (pLB1) | CIB strain containing plasmid pLB1 | This work |
| Azoarcus sp. CIBdbzdN (pLB1) | CIBdbzdN strain containing plasmid pLB1 | This work |
| A. communis SWub3 (pLB1) | SWub3 strain containing plasmid pLB1 | This work |
| Plasmids | ||
| pECOR8 | Apr, pUC19 harbouring a 5.4 Kb EcoRI DNA fragment from Azoarcus sp. CIB that contains the right end of the bzd cluster | López‐Barragán et al., (2004) |
| pIZ1016 | Gmr, pBBR1MCS‐5 broad‐host range cloning vector | Moreno‐Ruiz et al., (2003) |
| pIZ1016* | Gmr, pIZ1016 derivative without the NcoI restriction site in the polylinker | This work |
| pIZECO | Gmr, 11.4 Kb‐pIZ1016* derivative harbouring the right end of the bzd cluster | This work |
| pLB1 | Gmr, 25.6 Kb‐pIZ1016* derivative harbouring the complete bzd cassette | This work |