Table 2.
Published examples of improvement of cellulase production in Trichoderma reesei by genetic engineering
| Gene | Manipulation method | Promoter | References |
|---|---|---|---|
| xyr1 | Constitutive overexpression | pki1 | Seiboth, Karimi et al. (2012) |
| Constitutive overexpression | tcu1 | Lv et al. (2015) | |
| Constitutive overexpression, and ace1 downregulation | pdc1, downregulation by antisense with the same promoter | Wang et al. (2014) | |
| cbh1 | Promoter engineering | CRE1 binding sites exchanged against ACE2 and HAP2/3/5 binding sites | Zou et al. (2012) |
| cre1 | Deletion | Nakari‐Setälä et al. (2009) | |
| Truncation | Nakari‐Setälä et al. (2009); Mello‐de‐Sousa et al. (2014) | ||
| ace1 | Deletion | Aro et al. (2003) | |
| ace3 | Introduction of multiple gene copies | Häkkinen et al. (2014) | |
| lae1 | Constitutive overexpression | gpd1 | Seiboth‐Karimi et al. (2012) |
| vel1 | Constitutive overexpression | tef1 | Karimi Aghcheh et al. (2014) |