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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Kidney Int. 2017 May 3;92(4):836–849. doi: 10.1016/j.kint.2017.03.005

Figure 3. Ultrastructural changes of the glomerulus after npnt knockdown by morpholino or miR-378a-3p in zebrafish larvae.

Figure 3

Figure 3

Transmission electron microscopy pictures and quantification of podocyte effacement (A) and widening of the lamina rara interna of the GBM (B) in zebrafish larvae 120 hpf. Normal, partial and complete effacement of podocytes is compared between WT zebrafish and zebrafish that were injected with npnt-MO (100 μM), CTRL-MO (100 μM), miR-378a-3p mimic (25 μM) or miR-CTRL (25 μM) in one to four cell stage or in the cardinal vein of the zebrafish at 48 hpf (c.v. miR-378a-3p (25 μM), c.v. miR-CTRL (25 μM)). Podocyte layer was analysed over a length of 91 μm for WT, 98 μm for miR-378a-3p, 86 μm for miR-CTRL, 104 μm for npnt-MO, 80 μm for CTRL-MO, 78 μm for c.v. miR-378a-3p and 84 μm for c.v. miR-CTRL. Scale bar = 500 nm.

C.v.: cardinal vein injection at 48 hpf; hpf: hours post fertilization. Bars indicate quantification of results.