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. Author manuscript; available in PMC: 2018 Oct 1.

Published in final edited form as: Kidney Int. 2017 May 3;92(4):836–849. doi: 10.1016/j.kint.2017.03.005

(A) Immunofluorescence double staining for NPNT (red; a, a′) and collagen IV (green; b, b′) as well as NPNT (red; d, d′) and podocalyxin (PODXL, green; e, e′) on human nephrectomy. Overlaps of both staining are shown in the merged pictures in yellow (c, c′, f, f′); scale bar = 50 μm.

In situ hybridization for miR-378a-3p (B) and immunohistochemistry staining for NPNT (C) on kidney biopsies from patients with different glomerular diseases. The lower panels depict quantification of miR-378a-3p and NPNT-positive area. Each triangle represents analysis of one glomerulus. * p< 0.05, ** p<0.01, *** p<0.001, n.s. not significant. Scale bar = 50 μm.

(A) Immunofluorescence double staining for NPNT (red; a, a′) and collagen IV (green; b, b′) as well as NPNT (red; d, d′) and podocalyxin (PODXL, green; e, e′) on human nephrectomy. Overlaps of both staining are shown in the merged pictures in yellow (c, c′, f, f′); scale bar = 50 μm.

In situ hybridization for miR-378a-3p (B) and immunohistochemistry staining for NPNT (C) on kidney biopsies from patients with different glomerular diseases. The lower panels depict quantification of miR-378a-3p and NPNT-positive area. Each triangle represents analysis of one glomerulus. * p< 0.05, ** p<0.01, *** p<0.001, n.s. not significant. Scale bar = 50 μm.