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. 2017 Jun 26;36(42):5910–5913. doi: 10.1038/onc.2017.211

Figure 4.

Figure 4

(a) Cells depleted of OPRM1 are insensitive to methadone addition. Cells infected with pRS and pRS-shOPRM1 were treated with methadone and/or L-asparaginase for 4 days before cell viability assay. **P<0.05. (b) Increased sensitivity of TIB202 (▪), SEM (◊), MOLT3 (♦), C1 (□) and model leukemic (○) cells compared to model leukemic cells depleted of OPRM1 (•). Cells were treated with increasing concentrations of L-asparaginase for 4 days before cell viability assay. (c) Reduced level of OPRM1 is linked to L-asparaginase resistance in leukemic cells isolated from ALL patients. Peripheral blood samples were obtained from children diagnosed with leukemia at the Alberta Children’s Hospital following local ethics board approval and parental consent (REB815-1383-REN1). A panel of primary leukemia cells were treated with increasing concentrations of L-asparaginase before cell viability assay. The ratios of the OPRM1 vs actin band intensities were determined following densitometric scanning of bands using the NIH Image J 1.61 software (Bethesda, MD, USA). Values in A and B are means±s.d. of three independent experiments.