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. 2017 Jun 19;36(42):5861–5873. doi: 10.1038/onc.2017.182

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Expression of miR16 and its target genes in human GBM cell lines. (a) The levels of miR16 and mRNA of its target genes in glioblastoma cells SGH44, U87, U251 and their stem cell counterparts were evaluated by analysis of qRT–PCR. Comparison of the difference of miR16 expression between groups of miR16 inhibitor and negative control (NC) was performed. Comparison of the difference of mRNA expression of miR16 target genes, including Bcl2, CDK6, CCND1, CCNE1 and SOX5 between groups of miR16 mimic or inhibitor or NC was also performed. (b) Protein level of miR16 targeted genes: Bcl2, CDK6, CCND1, CCNE1 and SOX5 in groups of miR16 mimic or inhibitor or NC were determined by western blot analysis.