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. 2017 Jun 19;36(42):5885–5896. doi: 10.1038/onc.2017.194

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Copyright © 2017 The Author(s)

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Induction of morphological changes of the tumors by mutant p53^R270H. (a) Representative photographs of the indicated genotype organoids. Bars, 1 mm. The ratios of morphological classifications of Apc^Δ716 Trp53^R270H/R270H organoids are shown as a bar graph (mean±s.d.) (bottom right). (b) Representative confocal microscopy images of Apc^Δ716 Trp53^+/+ (left), Apc^Δ716 Trp53^flox/flox (center) and Apc^Δ716 Trp53^R270H/R270H tumor organoids (right). Fluorescence immunostaining for p53 (green, top) and double immunostaining for p53 and EdU (green and red, respectively, bottom) with DAPI staining (blue). The insets show enlarged images. Bars, 100 μm. (c) The EdU labeling index of tumor organoids is shown (mean±s.d.). ns, not significant. (d) A schematic diagram of the representative patterns of intestinal tumor branching leading to tube formation (top). The ratio of the branching number classification of Apc^Δ716 Trp53^+/+, Apc^Δ716 Trp53^flox/flox and Apc^Δ716 Trp53^+/R270H intestinal tumors are shown (bottom). Each dot represents the average rate (%) of the branching number in the individual mouse tumors. (e) Representative micrographs of human CRC with TP53 wild-type and R273H mutation (left, H&E). Bars, 200μm. Branching scores of human CRC with TP53 mutations around codon 273 (red, yellow and orange) compared with TP53 wild-type cases (blue) are shown (right). Asterisk, P<0.05.