Table I.
Gene | Upstream primer | Downstream primer | Amplified fragment (bp) |
---|---|---|---|
TYR | 5′TCACGGCTCTGTTGAATGTCT3′ | 5′CTGAAGTTGGGCGAGATGAT3′ | 300 |
TRP-1 | 5′ACATCATTCCCTCACCAAAGAC3′ | 5′AGAAGTCCGAAAGCCAAGTAAA3′ | 303 |
TRP-2 | 5′GTTCCTTTCTTCCCTCCAGTG3′ | 5′TTCCTTTATTGTCAGCGTCAG3′ | 300 |
ERK1 | 5′GGGAGGTGGAGATGGTGAAG3′ | 5′AGCAGGTTGGAGGGCTTTAGAT3′ | 441 |
ERK2 | 5′ACCCACACAAGAGGATTGAAGT3 | 5′AAAAGCCACAACTACCAGAAAC3′ | 353 |
JNK2 | 5′CCTTCTTTACCAGATGCTTTGTG3′ | 5′ATACGGTCAGTGCCTTGGAATA3′ | 303 |
β-actin | 5′CGTGGACATCCGCAAAGAC3′ | 5′AAGAAAGGGTGTAACGCAACTA3′ | 302 |
TYR, tyrosinase; TRP-1, tyrosinase related protein 1; TRP-2, tyrosinase related protein 2; ERK1, extracellular signal-regulated kinase 1; ERK2, extracellular signal-regulated kinase 2; JNK2, c-Jun N-terminal kinase 2.