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. 2017 Sep 1;14(5):4272–4278. doi: 10.3892/etm.2017.5089

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Copyright: © Tan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of PHC on the expression of apoptosis-related proteins. Following 3 h reperfusion, the expression of apoptosis-related proteins was analyzed using western blotting. Band densities were normalized to β-actin or COX IV. (A) Bax protein expression. (B) Bcl-2 protein expression. (C) VDAC1 protein expression. (D) Cytosol cyt-c protein expression. (E) Cleaved caspase-3 protein expression. Data are expressed as mean ± standard deviation (n=6). ^##P<0.01 vs. sham; **P<0.01 vs. I/R. ns, not significant (P>0.05); I/R, ischemia/reperfusion; PHC, penehyclidine hydrochloride; COX IV, cytochrome c oxidase subunit 4; VDAC1, voltage dependent-selective anion channel protein 1; cyt-c, cytochrome-c.