Table I.
Toxins A and B primer and MLST of 7 housekeeping genes.
| Toxin | Gene | Direction | Sequence (5′-3′) | Fragment size (bp) |
|---|---|---|---|---|
| tcdA | tcdA | F | AGATTCCTATATTTACATGACAATAT | 369 |
| R | GTATCAGGCATAAAGTAATATA CTTT | |||
| tcdB | NK104 | F | GTGTAGCAATGAAAGTCCAAGTTTACGC | 204 |
| NK105 | R | CACTTAGCTCTTTGATTGCTGCACCT | ||
| Adk | F | TTACTTGGACCTCCAGGTGC | 635 | |
| R | TTTCCACTTCCTAAGGCTGC | |||
| MSLT | atpA | F | TGATGATTTAAGTAAACAAGCTG | 674 |
| R | AATCATGAGTGAAGTCTTCTCC | |||
| dxr | F | GCTACTTTCCATTCTATCTG | 525 | |
| R | CCAACTCTTTGTGCTATAAA | |||
| glyA | F | ATAGCTGATGAGGTTGGAGC | 625 | |
| R | TTCTAGCCTTAGATTCTTCATC | |||
| recA | F | CAGTAATGAAATTGGGAGAAGC | 705 | |
| R | ATTCAGCTTGCTTAAATGGTG | |||
| sodA | F | CCAGTTGTCAATGTATTCATTTC | 585 | |
| R | ATAACTTCATTTGCTTTTACACC | |||
| tpi | F | ATGAGAAAACCTATAATTGCAG | 640 | |
| R | TTGAAGGTTTAACACTTCCACC |
tcd, Clostridium difficile toxin; F, forward; R, reverse; Adk, adenosine kinase; MSLT, multilocus sequence typing; atpA, ATP synthase subunit alpha; dxr, 1-deoxy-D-xylulose 5-phosphate reductoisomerase; glyA, serine hydroxymethyltransferase; soda, superoxide dismutase; tpi, triosephosphate isomerase.