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. 2017 Oct 11;6(10):e89. doi: 10.1038/emi.2017.79

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Copyright © 2017 The Author(s)

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Bio-panning of anti-ZIKV DIII protein Fabs from the full-human Fab library. (A) The individual E protein (black oval), which consists of DI, DII and DIII, is indicated. DI, DII and DIII are shown in red, yellow, and blue, respectively. (B) Polyclonal phage ELISA showing the phage enrichment of four rounds (Rounds 1–4) by panning. (C) Binding of Fabs m301–m312 to ZIKV DIII protein according to ELISA. (D) Western blot analysis of binding activity for Fabs m301–m304. Domain, D; enzyme-linked immunosorbent assay, ELISA; Zika virus, ZIKV.