Fig. 4.

Sirtinol regulates Th17/Treg balance. a Naive CD4⁺ T cells were isolated from mouse spleen, and polarized for Th17 differentiation in the presence different concentration of sirtinol. IL-17A expressing CD4⁺ T cells were measured by flow cytometry. Shown are representative of five separate experiments. b Percentages of IL-17A⁺ cells were presented. c The effect of sirtinol on the expressions of RORγt and Foxp3 measured by western blots. Shown are representative of five separate experiments. d–f Gene expressions of IL-17A, RORγt, Foxp3 in CD4⁺ T cells measured by qPCR. The data are expressed as mean ± SD (n = 5). *P < 0.05, **P < 0.01, compared with DMSO group