Table 1.

Oligonucleotide primers used to amplify and sequence the rbcL, matK, and ITS markers and their associated PCR thermocycling regimes

Marker	Primer	Source	PCR conditions
rbcL	aF	Pryer et al., 2001 [55]	5 min of initial denaturation at 80 °C; 30 cycles: 95 °C 1 min, 60 °C 1 min, 65 °C 2 min; final extension 5 min
	1379R	Pryer et al., 2001 [55]
matK	matK-xf	Ford et al., 2009 [56]	5 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
	matK-MALP	Dunning and Savolainen, 2010 [57]
ITS	ITS5a	Stanford et al., 2000 [58]	6 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
	ITS4	White et al., 1990 [59]