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Itt1p interacts with both eRF3 and eRF1. All proteins were isolated from E. coli, except eRF3 (S.c.), isolated from S. cerevisiae. The indicated proteins were incubated with GST-Itt1p or GST proteins immobilized on glutathione-Sepharose 4B. Following washing, bound proteins were eluted and analyzed by Western blotting with polyclonal antibodies against eRF3 and eRF1.
