Fig. 7.

miR-92b-3p/Gabra3 interaction regulates PC cell proliferation and invasion via the AKT/m-TOR and JNK pathways. a Clinical specimens of normal pancreatic epithelium and PC with low and high TNM stages were stained for miR-92b-3p, Gabra3, Ki-67, p-p70s6k, MMP-2 and MMP-9. Representative images from a tissue microarray are shown. Original magnification, ×400; Scale bars: 50 μm. b Association analyses of miR-92b-3p levels and the number of Ki-67⁺ cells and the levels of p-p70s6k, MMP-2 and MMP-9 in 82 FFPE PC tissues. c Immunoblotting analyses of the levels of proteins in the AKT/m-TOR and JNK pathways in PC cells after transfection with miR-92b-3p mimic, inhibitor, or negative control. d-e MMP-2 and MMP-9 activities were measured by gelatin zymography in AsPC-1 and SW1990 cells after transfection with miR-92b-3p mimic, inhibitor, or negative control. f Immunoblotting analyses to detect p-p70s6k, MMP-2 and MMP-9 protein expression in PC cell lines co-transfected with the miR-92b-3p mimic and GABRA3 construct. g Gelatin zymography analyses to detect MMP-2 and MMP-9 activities in PC cell lines co-transfected with miR-92b-3p mimic and GABRA3 construct. All n = 3; bar, SEM; *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t test