Fig. 2. Dose–response curves of compounds 2, 3 and 4, before and after irradiation, obtained by measuring the LasQS controlled bioluminescence at different concentrations, using the E. coli JM109 pSB1075 sensor strain. Dose–response curve of non-irradiated (black) and λ = 365 nm light-irradiated (red) compounds 2 (a), 3 (b) and 4 (c). (d) and (e) Effect of photoswitching on the biological activity of compounds 2 (d) and 3 (e). Both compounds were used at a concentration of 6.25 μM for the sequential rounds of photoirradiation. Data points and error bars represent the mean and pooled standard deviation of two biological replicates each performed in triplicate.
