| Antibodies |
| PTPN14/Pez (F-12) antibody |
Santa Cruz |
sc-373766 |
| PTPN14/Pez (H-67) antibody |
Santa Cruz |
sc-68384 |
| PTPN14/Pez antibody |
Laboratory of Dr. Yeesim Khew-Goodall |
N/A |
| YAP (D8H1X) XP® rabbit mAb |
Cell signaling |
14074 |
| p53 antibody (CM5) |
Leica (Novocastra) |
P53-CM5P |
| BrdU antibody |
Becton Dickinson |
555627 |
| CD133-APC antibody |
eBioscience |
17-1331-81 |
| CD45-eFluor 450 antibody |
eBioscience |
48-0451-82 |
| Ter-119-eFluor 450 antibody |
eBioscience |
48-5921-82 |
| Ck19 antibody |
University of Iowa (DSHB) |
TROMAIII |
| Gapdh antibody |
Fitzgerald |
10R-G109a |
| Actb antibody |
Santa Cruz |
sc-1615 |
| HA tag monoclonal antibody (2–2.2.14) |
ThermoFisher Scientific |
26183 |
| Mdm2 antibody (#2A10) |
Abcam |
ab16895 |
| Muc5ac antibody |
ThermoFisher Scientific |
145-P1 |
| V5 Epitope tag antibody |
Novus Biological |
NB600-381 |
| Fluorescein goat anti-rabbit IgG |
Vector Laboratories |
FI-1000 |
| Alexa Fluor 546 goat anti-mouse IgG |
Invitrogen |
A-11003 |
| Biotinylated goat anti-rat |
Vector Laboratories |
BA-9401 |
| Biotinylated goat anti-mouse |
Vector Laboratories |
BA-9200 |
| Biotinylated goat anti-rabbit |
Vector Laboratories |
BA-1000 |
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| Bacterial and Virus Strains |
| Ad-Cre or Ad5CMVCre |
University of Iowa |
VVC-U of Iowa-5 |
| Ad-empty or Ad5CMVempty |
University of Iowa |
VVC-U of Iowa-272 |
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| Biological Samples |
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| Tissue microarrays |
Laboratory of Dr. Albert Koong |
N/A |
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| Chemicals, Peptides, and Recombinant Proteins |
| Verteporfin |
Fisher Scientific |
530150 |
| Doxorubicin hydrochloride |
Sigma |
D1515 |
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| Critical Commercial Assays |
| Illumina TruSeq Kit (v.2) |
Illumina |
RS-122-2001 |
| VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) |
Vector Laboratories |
PK-6100 |
| DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3′-diaminobenzidine |
Vector Laboratories |
SK-4100 |
| ChromPure Goat IgG, whole molecule |
Jackson Immunoresearch Laboratories |
005-000-003 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit |
Invitrogen |
L-34965 |
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| Deposited Data |
| Mouse primary PanINs |
This paper |
GSE94566 |
| HrasV12-expressing MEFs homozygous for different Trp53 alleles |
Brady et al., 2011 |
GSE27901 |
| Mouse PDAC gene expression |
Collisson et al., 2011 |
GSE17891 |
| Mouse p53 CHIP-seq |
Kenzelmann-Broz et al., 2013 |
GSE46240 |
| Human p53 CHIP-seq |
Younger et al., 2015 |
GSE55727 |
| TCGA PANCAN12 |
Synapse |
https://www.synapse.org/#!Synapse:syn300013/wiki/70804 |
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| Experimental Models: Cell Lines |
| Mouse: KPC pancreatic cancer cell line: Kras+/LSL- G12D;Pdx1-Cre;Trp53fl/fl
|
Laboratory of Dr. Nabeel Bardeesy |
NB490 |
| Mouse: KIC pancreatic cancer cell line: Kras+/LSL- G12D;Pdx1-Cre;Cdkn2afl/fl
|
Laboratory of Dr. Nabeel Bardeesy |
32a and 33 |
| Mouse: p53WT non-small cell lung cancer cell line: KrasLA2/+;Trp53LSL-WT/LSL-WT
|
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: p5325,26 non-small cell lung cancer cell line: KrasLA2/+;Trp53LSL-25,26/LSL-25,26
|
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: p5353,54 non-small cell lung cancer cell line: KrasLA2/+;Trp53LSL-53,54/LSL-53,54
|
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: p5325,26,53,54 non-small cell lung cancer cell line: KrasLA2/+;Trp53LSL-25,26,53,54/LSL-25,26,53,54
|
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: Eμ-Myc B cell lymphoma cells: Eμ-myc;Trp53ER/−
|
Laboratory of Dr. Lin He |
N/A |
| Human: Panc-1 pancreatic cancer cell line: TP53 MUT |
ATCC |
CRL-1469 |
| Human: MIA PaCa-2 pancreatic cancer cell line: TP53 WT |
ATCC |
CRL-1420 |
| Human: Capan 2 pancreatic cancer cell line: TP53 MUT |
ATCC |
HTB-80 |
| Human: HCT116 p53 WT colorectal cancer cell line: TP53 WT |
Laboratory of Dr. Bert Vogelstein |
N/A |
| Human: HCT116 p53 MUT colorectal cancer cell line: TP53−/−
|
Laboratory of Dr. Bert Vogelstein |
N/A |
| Human: A549 lung cancer cell line: TP53 WT |
Stanford University School of Medicine laboratories |
N/A |
| Human: H1299 lung cancer cell line: TP53 MUT |
Stanford University School of Medicine laboratories |
N/A |
| Human: MCF7 breast cancer cell line: TP53 WT |
Stanford University School of Medicine laboratories |
N/A |
| Human: MDA-MB-231 breast cancer cell line: TP53 MUT |
Stanford University School of Medicine laboratories |
N/A |
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| Experimental Models: Organisms/Strains |
| Mouse: Trp53flox mice: B6.129P2-Trp53tm1Brn/J |
The Jackson Laboratory |
008462 |
| Mouse: Trp53 null− mice: B6.FVB-Tg(Pdx1-cre)6Tuv/Nci |
Laboratory of Dr. Tyler Jacks |
N/A |
| Mouse: Trp53LSL-53,54 mice: 129-Trp53tm3Att/J |
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: Trp53LSL-25,26,53,54 mice: 129-Trp53tm4Att/J |
Laboratory of Dr. Laura Attardi |
N/A |
| Mouse: Trp53LSL-wt mice: B6.129S4-Trp53tm5Tyj/J |
Laboratory of Dr. Tyler Jacks |
N/A |
| Mouse: KrasLSL-G12D mice: B6.129S4-Krastm4Tyj/J |
Laboratory of Dr. Tyler Jacks |
N/A |
| Mouse: Pdx1-Cre mice: B6.FVB-Tg(Pdx1-cre)6Tuv/J |
NCI Mouse Repository |
01XL5 |
| Mouse: Ptf1a-Cre mice: 129-Ptf1atm1.1(cre)Cvw/J |
Laboratory of Dr. Christopher Wright |
N/A |
| Mouse: ICR/Scid mice: IcrTac:ICR-Prkdcscid
|
Taconic Biosciences |
ICRSC-M |
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| Oligonucleotides |
| See table S4 for primers |
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| Recombinant DNA |
| pMGIB-3xHA- |
Laboratory of Dr. Steven Artandi |
N/A |
| pMGIB-3xHA-p53 |
Laboratory of Dr. Laura Attardi |
N/A |
| pMGIB-3xHA-Ptpn14 |
This paper |
N/A |
| pCDNA3.1-3XHA-Ptpn14 |
This paper |
N/A |
| pCDNA3.1-3XHA-Ptpn14-C1121S |
This paper |
N/A |
| pCDNA3-V5-Ptpn14-del-N |
Addgene |
61004 |
| pCDNA3-V5-Ptpn14-del-C |
Addgene |
61005 |
| pCDNA3-V5-Ptpn14-PPxY |
Addgene |
61006 |
| LPE-Ren_713 |
Mirimus |
713 |
| LPE-Trp53_1224 |
Mirimus |
1224 |
| LPE-Ptpn14_8252 (shPtpn14-1) |
Mirimus |
19250 |
| LPE-Ptpn14_10201 (shPtpn14-2) |
Mirimus |
19250 |
| pLKO-shYap1 |
Addgene |
42540 |
| pLKO-shLacZ |
Addgene |
42559 |
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| Software and Algorithms |
| ImageJ |
NIH |
https://imagej-nih-gov.laneproxy.stanford.edu/ij/ |
| GSEA |
Broad Institute |
http://software.broadinstitute.org/gsea/index.jsp |
| DISCOVER package |
Canisius et al., 2016 |
http://ccb.nki.nl/software/discover/ |
| ComplexHeatmap |
Gu et al., 2016 |
http://www.bioconductor.org/packages/devel/bioc/html/ComplexHeatmap.html |
| Survcomp |
Schroder et al., 2011 |
http://bioconductor.org/packages/release/bioc/html/survcomp.html |
| OpenCFU |
Geissmann, 2013 |
http://opencfu.sourceforge.net/ |
| RNA Express Legacy; version: 1.0.0 (Basespace) |
Illumina, Inc. |
https://basespace.illumina.com |
| Adobe Photoshop CS6 |
Adobe |
Version 13.0.1 x64 |
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| Other |
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