Table 1. Conversions and regioselectivity of PyrH and PrnA with 1–4.

Substrate	Enzyme	Conversion a	Product b (o/p-ratio)
1	PyrH	100%	1a (p-100%)
	PrnA	59%	1b (o-100%)
2	PyrH	67%	2a (p-100%)
	PrnA	76%	2b (o-100%)
3	PyrH	46%	3a (p-100%)
	PrnA	19%	3a (p-86%), 3b (o-14%)
4	PyrH	<1%	4a (p-100%)
	PrnA	1%	4a (p-16%), 4b (o-84%)

^aConversion after 30 minutes with halogenase enzyme (10 μM) and substrate (0.5 mM).

^bRatios of halogenation ortho or para to the aryl NH/NH₂ group was determined by HPLC.