Table 2.

Percentage yield of thiol-labeled RNA from hippocampal neurons after subcutaneous administration of 4TU

Sample type	Age	Tissue	Percent yield
TetO-UPRT; CaMKII-tTA	P50	Hippocampus	3.90%
TetO-UPRT; CaMKII-tTA	P90	Hippocampus	3.20%
TetO-UPRT	P120	Hippocampus	2.00%
TetO-UPRT	P120	Hippocampus	2.00%
WT	P50	Hippocampus	0.07%
WT	P90	Hippocampus	0.10%

CaMKII-tTA; TetO-UPRT double positive mice, TetO-UPRT single positive mice, or WT control mice were injected subcutaneously with 4TU and hippocampus was removed 5 h later. After RNA extraction and streptavidin purification, the amount of tagged RNA obtained was much higher from hippocampal samples expressing UPRT in neurons (CaMKII-tTA driven neuronal expression and leaky neuronal expression in single positive TetO-UPRT mice; Fig. 6-2).