Table 2.
Electrophysiological and anatomic characteristics for all four interneuron clusters with statistical comparisons
| Cluster | 1 | 2 | 3 | 4 | 1,2 | 1,3 | 1,4 | 2,3 | 2,4 | 3,4 |
|---|---|---|---|---|---|---|---|---|---|---|
| n | 30 | 29 | 16 | 31 | ||||||
| Input resistance (MΩ) | 220.2 | 85.7 | 274.4 | 155.1 | ||||||
| 12.3 | 5.1 | 14.9 | 6.2 | p < 0.01 | n.s. | p < 0.05 | p < 0.01 | p < 0.01 | p < 0.01 | |
| Time constant, falling (ms) | 13.7 | 5.1 | 15.8 | 8.9 | ||||||
| 0.8 | 0.2 | 0.9 | 0.4 | p < 0.01 | n.s. | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 | |
| Time constant, rising (ms) | 14.1 | 5.2 | 16.2 | 9.5 | ||||||
| 0.8 | 0.2 | 1.0 | 0.4 | p < 0.01 | n.s. | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 | |
| Rebound amplitude (mV) | 0.75 | 0.41 | 1.48 | 0.62 | ||||||
| 0.08 | 0.03 | 0.20 | 0.10 | p < 0.05 | n.s. | n.s. | p < 0.01 | n.s. | p < 0.01 | |
| Firing threshold (mV) | −38.7 | −36.7 | −40.8 | −35.6 | ||||||
| 0.9 | 1.0 | 1.1 | 1.0 | n.s. | n.s. | n.s. | p < 0.05 | n.s. | p < 0.05 | |
| Resting membrane potential (mV) | −66.3 | −71.4 | −55.5 | −66.8 | ||||||
| 1.0 | 1.0 | 2.1 | 1.1 | p < 0.01 | p < 0.01 | n.s. | p < 0.01 | p < 0.05 | p < 0.01 | |
| A.P. rise time (ms) | 0.273 | 0.192 | 0.253 | 0.292 | ||||||
| 0.006 | 0.003 | 0.013 | 0.009 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.01 | n.s. | |
| A.P. half-width (ms) | 1.085 | 0.526 | 0.832 | 1.151 | ||||||
| 0.038 | 0.010 | 0.067 | 0.047 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.01 | p < 0.05 | |
| Spike AHP (mV) | 16.2 | 19.9 | 19.3 | 20.8 | ||||||
| 1.0 | 0.5 | 1.0 | 0.7 | p < 0.05 | n.s. | p < 0.01 | n.s. | n.s. | n.s. | |
| F-I gain (Hz/nA) | 153.0 | 281.9 | 373.9 | 162.0 | ||||||
| 18.9 | 15.8 | 51.4 | 13.0 | p < 0.01 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.01 | |
| Peak firing rate (Hz) | 57.4 | 279.2 | 111.5 | 75.3 | ||||||
| 5.1 | 8.6 | 8.4 | 5.6 | p < 0.01 | p < 0.01 | n.s. | p < 0.01 | p < 0.01 | n.s. | |
| Lowest firing rate (Hz) | 10.6 | 79.2 | 13.7 | 13.9 | ||||||
| 2.3 | 6.0 | 3.0 | 2.3 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.01 | n.s. | |
| Rheobase (pA) | 185.7 | 380.7 | 118.8 | 262.6 | ||||||
| 15.7 | 22.2 | 12.3 | 13.2 | p < 0.01 | n.s. | p < 0.05 | p < 0.01 | p < 0.05 | p < 0.01 | |
| Adaptation ratio | 0.783 | 0.878 | 1.335 | 0.917 | ||||||
| 0.153 | 0.007 | 0.730 | 0.153 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.05 | n.s. | |
| Sag ratio | 0.936 | 0.945 | 0.888 | 0.936 | ||||||
| 0.006 | 0.004 | 0.013 | 0.008 | n.s. | p < 0.05 | n.s. | p < 0.01 | n.s. | p < 0.01 | |
| Change in impedance (%) | 64.8 | 133.1 | 136.5 | 51.6 | ||||||
| 10.6 | 12.7 | 15.7 | 9.2 | p < 0.01 | p < 0.01 | n.s. | n.s. | p < 0.01 | p < 0.01 | |
| Soma depth (µm) | 327.0 | 304.9 | 274.6 | 191.6 | ||||||
| 14.2 | 16.1 | 19.7 | 8.9 | n.s. | n.s. | p < 0.01 | n.s. | p < 0.01 | p < 0.05 | |
| Axonal tree, superficial (µm) | 296.4 | 168.4 | 107.0 | 114.0 | ||||||
| 21.5 | 19.8 | 33.2 | 25.2 | p < 0.01 | p < 0.01 | p < 0.01 | n.s. | n.s. | n.s. | |
| Axonal tree, deep (µm) | 446.4 | 298.0 | 318.0 | 252.9 | ||||||
| 23.0 | 16.4 | 35.6 | 25.7 | p < 0.01 | p < 0.05 | p < 0.01 | n.s. | n.s. | n.s. | |
| Axonal tree, width (µm) | 361.4 | 513.0 | 440.6 | 472.2 | ||||||
| 21.7 | 12.8 | 30.8 | 20.1 | p < 0.01 | n.s. | p < 0.01 | n.s. | n.s. | n.s. |
The electrophysiological and anatomic characteristics of all four clusters are shown, along with the associated p values from a Kruskal--Wallis test (as described in Materials and Methods). Each row shows the average value for a different electrophysiological or anatomic measurement. The standard error associated with that measurement is located below the average value. Each column for the left half of the table shows the measurements for each of the four clusters. On the right half of the table, the p value for the Kruskal--Wallis test is shown for matched pairs. For example, column “1,2” shows the p values for the test between clusters 1 and 2 for each characteristics; p < 0.05 are highlighted in blue. Tests that showed no significance are shown as n.s.