Figure 3. In vivo signaling by non-γc cytokines is required for generation of γc-independent CD8 T cells.

(a,b) SP8 thymocyte frequencies (top) and numbers (bottom) in γc^cKO mice (red bars) were compared with those in γc^cKO mice additionally deficient in cytokine and/or cytokine receptor genes (other colored bars). Numbers within bars display SP8 cell frequencies and numbers relative to those in normal B6 mice (set equal to 100%). For simplicity, γc^cKOIL6^KOIFN-γR^KOTSLPR^KO mice are designated CytoQuad mice. Data are from 4-50 combined mice from 2-39 experiments. (c) Thymocyte profiles from the indicated mice are shown with total thymocyte numbers indicated. Numbers within or adjacent to boxes in the profiles indicate frequency of cells in that box. Data are from 8-13 mice combined from 5 experiments. (d) SP8 thymocyte frequencies (left) and numbers (right) in the indicated mice were compared and are shown relative to B6 which was set equal to 100%. Data are from 13-50 mice combined from 7-39 experiments. Mean and s.e.m. are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.