Table 2. Rhizobial isolates from bean plants and their relevant characters.
Isolatea | nifHb | RNA RFLPc | Growth in TY with NaCl (%) | Growth in TY medium at pH | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
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Pattern | Type | 0.5 | 1.0 | 1.5 | 2.0 | 2.5 | 4.5 | 5.0 | 5.5 | 8.0 | 8.5 | 9.0 | ||
R. leguminosarum–like group (22) | ||||||||||||||
FH14 | + | + | + | + | + | + | + | + | + | + | + | + | ||
FH19 | + | + | + | + | + | + | + | + | + | + | + | + | ||
FH31 | + | W | − | − | − | − | − | + | + | − | − | − | ||
FH32 | + | + | − | − | − | − | W | + | + | + | + | W | ||
FH20 | + | + | − | − | − | − | + | + | + | + | − | − | ||
FH23 | + | + | W | − | − | − | + | + | + | + | + | W | ||
FH25 | + | + | + | − | − | − | − | + | + | + | + | + | ||
FH27 | + | + | + | − | − | − | − | + | + | + | + | + | ||
FH34 | + | + | − | − | − | − | W | + | + | + | W | W | ||
NH01 | + | + | W | − | − | − | W | + | + | + | W | − | ||
NH05 | + | + | W | − | − | − | − | + | + | + | W | − | ||
NH06 | + | AAAA | 1 | + | W | − | − | − | + | + | + | + | + | + |
NH07 | + | ND | ND | ND | ND | ND | − | − | ND | ND | ND | ND | ||
NH10 | + | + | − | − | − | − | − | + | + | + | + | − | ||
NH15 | + | + | − | − | − | − | − | + | + | + | + | − | ||
NH16 | + | + | − | − | − | − | − | + | + | + | + | + | ||
NH37 | + | + | W | W | − | − | − | + | + | + | + | + | ||
FH02 | ND | ++ | + | − | − | − | − | + | + | + | + | + | ||
FH08 | ND | ++ | W | − | − | − | − | + | + | + | + | + | ||
FH11 | ND | + | W | − | − | − | − | + | + | + | + | + | ||
FH12 | ND | + | W | − | − | − | − | + | + | + | + | + | ||
FH13 | ND | + | W | − | − | − | − | + | + | + | + | + | ||
R. grahamii (2) | ||||||||||||||
FH29-1 | + | AABA | 2 | + | − | − | − | − | + | + | + | + | + | + |
NH18 | + | AADA | 3 | + | + | − | − | − | W | + | + | + | + | − |
Ensifer americanum (15) | ||||||||||||||
FG01 | + | + | + | W | − | − | − | + | + | + | + | + | ||
FG04 | + | + | + | W | − | − | − | + | + | + | + | + | ||
FG08 | + | + | + | + | W | − | − | + | + | + | + | + | ||
FG03 | ND | + | + | + | W | − | − | + | + | + | + | + | ||
FG09 | + | + | + | W | W | − | − | + | + | + | + | + | ||
FG10 | + | EAAH | 4 | + | + | W | W | − | − | + | + | + | + | + |
NG13 | ND | + | + | + | W | − | − | + | + | + | + | + | ||
NG07A | + | + | + | + | − | − | − | + | + | + | + | + | ||
NG07B | + | + | + | + | W | − | − | + | + | + | + | + | ||
NG12 | + | + | + | + | − | − | − | + | + | + | + | + | ||
NG16 | + | + | + | + | − | − | − | + | + | + | + | + | ||
NG35 | ND | + | + | W | − | − | − | + | + | + | + | + | ||
FG17B | + | + | + | W | − | − | − | + | + | + | + | + | ||
FG20 | + | EADH | 5 | + | + | W | − | − | − | + | + | + | + | + |
FG25 | + | + | + | W | W | − | − | + | + | + | + | + | ||
Rhizobium radiobacter (2) | ||||||||||||||
NF20A | + | HADA | 6 | ++ | ++ | ++ | ++ | ++ | + | + | + | + | + | + |
NF22 | + | ++ | ++ | ++ | ++ | ++ | + | + | + | + | + | + | ||
Reference strains | ||||||||||||||
E. meliloti Rm1021 | + | + | + | + | + | − | + | + | + | + | + | |||
R. giardinii H152T | + | W | − | − | − | − | W | + | + | + | W | |||
R. gallicum USDA2918T | + | W | W | − | − | − | + | + | + | + | W | |||
R. leguminosarum USDA2370T | + | W | − | − | − | − | + | + | + | + | W | |||
R. etli CFN42T | + | W | − | − | − | − | + | + | + | + | + | |||
R. phaseoli ATCC14482T | + | + | − | − | − | − | + | + | + | + | + | |||
R. lusitanum LMG22705T | + | W | − | − | − | + | + | + | + | + | + |
+, growth; ++, vigorous growth; −, no growth; ND, not determined; W, weak growth.
The first letter in the isolate number represents the origin of the cultivar (e.g. F = Flor de Mayo and N = Negro Querétaro), whereas the second letter corresponds to the soil sample site (e.g. H = Hidalgo, G = General Cepeda, Coahuila, F=Parras de la Fuente, Coahuila). Boldface isolates were isolates used in sequence analysis.
The nifH gene was detected by PCR after isolation.
PCR-based RFLP of 16S rRNA gene digested seperately with the restriction endonucleases MspI, AluI, HaeIII and HinfI.