Table 3. Mobility data (diameter of colony in mm) in soft PY medium (0.4% agar) incubated at 30°Ca.
| Isolate | With NaClb | With pHc | Incubation time (h) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
||||||||||
| 0.5% | 1.0% | 1.5% | 4.5 | 5.0 | 6.0 | 7.0 | 8.0 | 8.5 | 9.0 | ||
| R. leguminosarum-like group | |||||||||||
| FH14 | 5.0 ± 0.0 | 6.0 ± 0.0 | 12.5 ±5.5 | 6.0 ±0.0 | 6.0 ± 1.0 | 23.3 ±1.5 | >40 | 22.0 ±2.0 | 12.0 ±3.0 | 14.5 ±4.5 | 48 |
| FH19 | 5.0 ± 0.5 | 8.0 ± 1.0 | 23.0 ±2.0 | 5.2 ±0.8 | 5.5 ± 0.5 | 30.0 ± 0.0 | >40 | 30.0 ±0.0 | 12.5 ±4.5 | 14.0 ±4.0 | 48 |
| FH20 | 4.0 ± 0.0 | 5.5 ± 0.5 | 16.0 ±6.0 | 4.0 ±0.0 | 4.5 ± 0.5 | 19.0 ± 1.0 | 27.5 ± 2.5 | 17.0±1.0 | 12.0 ±4.0 | 12.5 ±5.5 | 48 |
| FH23 | 7.0 ± 1.0 | 10.0± 1.0 | 29.0 ±3.0 | 6.5 ±0.5 | 5.5 ± 0.5 | >45.0 | >40 | 35.0 ±0.0 | 12.5 ±3.5 | 22.0 ± 2.0 | 48 |
| FH31 | 5.0 ± 0.5 | 7.0 ± 0.0 | 15.0±1.0 | 4.5 ±1.0 | 5.0 ± 0.0 | 21.0 ±0.0 | >40 | 25.0 ±1.0 | 12.5 ±5.5 | 14.5 ±4.5 | 48 |
| FH32 | 5.0 ±5.0 | 5.0 ± 0.0 | 12.5 ±2.5 | − | 4.0 ± 1.0 | 22.0 ± 1.0 | >40 | 27.0 ±1.0 | 12.5 ±0.5 | 16.5 ±2.5 | 48 |
| FH34 | 6.5 ±1.0 | 9.0 ± 0.0 | 30.5 ± 2.5 | 6.0 ±0.0 | 5.0 ± 0.0 | >40.0 | >40 | 37.01.0 | 15.5 ±2.5 | 24.5 ±3.5 | 48 |
| R. grahamii | |||||||||||
| FH29-1 | 5.5 ± 0.5 | 8.0 ± 1.0 | 21.0± 1.0 | 5.0 ±0.0 | 5.5 ±1.0 | 25.0 ± 0.0 | >40 | 28.0 ± 1.0 | 14.0±3.0 | 16.5 ±2.5 | 48 |
| E. americanum | |||||||||||
| FG01 | w | w | − | − | w | 9.5 ±1.5 | 11.0 ±0.0 | 6.0 ±0.0 | + | + | 36 |
| FG04 | w | w | − | − | w | + | 4.5 ± 0.5 | + | + | + | 36 |
| FG17B | w | w | − | − | w | 8.0 ± 0.0 | 9.0 ± 0.0 | 6.0 ±0.0 | + | + | 36 |
| FG20 | w | w | − | − | w | 9.5 ± 0.5 | 11.0 ±0.0 | 8.0 ±0.0 | + | + | 36 |
| FG25 | w | w | − | − | w | 6.0 ± 0.0 | 9.0 ± 0.0 | + | + | + | 36 |
| NG07A | w | w | − | − | w | 7.5 ±1.5 | 10.0 ±0.0 | 6.0 ±0.0 | + | + | 36 |
| NG12 | w | w | − | − | w | + | + | + | w | w | 36 |
| NG16 | w | w | − | − | w | 6.0 ± 0.0 | 8.0 ± 0.0 | 5.00.0 | w | w | 36 |
a
Data are mean ± variation, n = 2; W, weak mobility; −, no growth; +, grow without mobility.
b
2.0% NaCl was also tested, but no isolate grew under this condition.
c
At pH 10, the Rhizobium isolates grew and moved weakly, while no Ensifer isolate grew.