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. Author manuscript; available in PMC: 2017 Oct 27.
Published in final edited form as: Methods Mol Biol. 2012;844:15–30. doi: 10.1007/978-1-61779-527-5_2

Fig. 1.

Fig. 1

The checkerboard titration plate map: Capture and detection antibodies are titrated against each other over a three-log range of standard concentrations. All parameters are assayed in duplicate wells.* An additional titration for strep:HRP may be examined by alternating strep:HRP dilutions across rows.