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. Author manuscript; available in PMC: 2017 Oct 27.
Published in final edited form as: Methods Mol Biol. 2012;844:15–30. doi: 10.1007/978-1-61779-527-5_2

Fig. 3.

Fig. 3

A conceptual Illustration of the sequential ELISA. After the samples are subjected to one ELISA for cytokine 1, they are transferred back to a source plate for storage until they are used in the ELISA for cytokine 2, so on and so forth. The steps after the sample incubation are as normal for the particular ELISA adapted from ref. 6.