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. Author manuscript; available in PMC: 2018 Sep 13.
Published in final edited form as: Cell Host Microbe. 2017 Sep 13;22(3):330–342.e4. doi: 10.1016/j.chom.2017.08.002

Figure 4. RAB27b effectors MyRIP and MyosinVIIa are critical for bacterial expulsion.

Figure 4

(A) Bacterial expulsion from infected BECs transfected with control siRNA or siRNA targeting MyRIP. Knockdown efficiency is indicated by western blots. Error bars represent SEM. The experiments were repeated three times, where each experiment employed n=6 wells.

(B) Bacterial expulsion from infected BECs transfected with control siRNA or siRNA targeting Myosin Va or MyosinVila. Knockdown efficiency is indicated by western blots. Error bars represent SEM. The experiments were repeated three times, where each experiment employed n=6 wells.

(C) Immunofluorescence staining of infected BECs revealing the co-association of RAB27b (green) and MyosinVIIa (red) with UPEC (blue). The percentage of intracellular bacteria that stain positive for MyosinVIIa over total intracellular UPEC stained positive for RAB27b is indicated in parenthesis. n=3 slides Scalebar: 5 μm.

(D) Bacterial expulsion from infected BECs transfected with control siRNA or siRNA targeting MyosinVIIa or Dynein respectively, or combined siRNA targeting both MyosinVIIa and Dynein. Error bars represent SEM. The experiments were repeated three times, where each experiment employed n=6wells.

(E) Immunofluorescence staining of infected BECs revealing the co-association of MyosinVIIa (red) and Dynein (green) with UPEC (blue). The percentage of intracellular bacteria that stain positive for MyosinVIIa and Dynein (double positive) over total intracellular UPEC counted is indicated in parenthesis. n=3 slides Scale bar: 5 μm.