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. 2017 Oct 27;12(10):e0186786. doi: 10.1371/journal.pone.0186786

Fig 1. Schematic T-DNA map of pART27 RNAi cassettes of MYMIV-AC2, MYMIV-AC4 and MYMIV AC2+AC4 stack in sense and antisense orientation.

Fig 1

Abbreviations: LB, left border; RB, right border; CaMV 35SP: Cauliflower mosaic virus 35S promoter; OCS terminator: octopine synthase terminator, PDK intron: pyruvate dehydrogenase kinase intron, CaMV 35S poly-A, Cauliflower mosaic virus 35S terminator; NPTII: Neomycin phosphotransferase; Restriction enzyme NotI were used for cloning of all the three RNAi cassettes from the intermediate RNAi vector pKANNIBAL into the plant transformation binary vector pART27.