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. 2017 Oct 27;12(10):e0186786. doi: 10.1371/journal.pone.0186786

Fig 4. Northern blot analysis of cowpea transgenic lines overexpressing MYMIV-AC4, AC2 and AC2+AC4stacked RNAi constructs for the analysis of siRNA prior and after challenge with MYMIV.

Fig 4

Samples are indicated on the top of each lane (UC-Unchallenged). Lower panel shows the total RNA as a loading control. A. siRNA accumulation in AC4 overexpression lines; B. AC2 overexpression lines and C-D. AC2-AC4 stacked overexpression lines. AC4-197nt transcript was used as a probe for hybridization of MYMIV-AC4 and MYMIVAC2+AC4 overexpressing plants. AC2-186nt transcript was used as a probe for hybridization of MYMIV-AC2 and MYMIVAC2+AC4 overexpressing plants, 21nt indicated the siRNA markers.