Fig 6. Screening of Cowpea (Vigna unguiculata) transgenic lines in the T0 generation for resistance against MYMIV and molecular confirmation of viral DNA accumulation by RCA analysis followed by restriction digestion using DNA-A specific unique cutter MfeI.
A. Phenotype of a resistant AC2 and AC4 lines derived from the RNAi construct targeting the AC2-186 nts and AC4- 197 nts of MYMIV showing 100% protection (right) when compared with complete infection in control plants (left) when challenged with MYMIV agroinfectious viral dimers after 14th day of inoculation. B. 9 weeks post agro infiltration. C. The appearance of (~2.7 kb) after digestion with MfeI of DNA-A (lane-1) indicates the presence of MYMIV infection in WT plant. Lane AC2#1, AC2#2 and AC4#1 represents the transgenic lines. Lane marked M represents 10 Kb molecular mass marker.