Table 1. Summary of the transformation of 4-day-old cotyledonary node explants of Vigna unguiculata cv.

Pusa Komal co-cultivated with Agrobacterium tumefaciens EHA105 harbouring MYMIV RNAi-AC2 construct.

Exp. no.	No. of explants inoculated in Agrobacterium suspension	No of shoot recovered on selection medium	Number of plants rooted	No. of plants positive for AC2 and nptII genes by PCR	Transformation efficiencya (%)
1	84	48	9	3	3.57
2	73	41	10	2	2.73
3	65	52	6	3	4.61
4	52	46	5	2	3.84
5	72	47	5	2	2.77
Total /average	346b	46.8c	35	12b	3.50c

^a Number of plants PCR positive for nptII, and AC2 per number of explants co-cultivated

^b Total

^c Average response