Table 3. Virus resistance assay performed for T1 transgenic cowpea plants challenged with agroinfectious clones of Mungbean yellow mosaic India virus (MYMIV).
Constructs | Progeny of T0 transgenic lines | Number of non- symptomatic plants and RCA negative/number of plants challenged | Type of symptoms |
---|---|---|---|
MYMIV-AC2 | Line-1 | 4/5 | No symptoms |
Line-3 | 5/5 | No symptoms | |
Line-7 | 4/5 | Mild mossaic | |
MYMIV- AC4 | Line-2 | 4/5 | No symptoms |
Line-4 | 2/5 | Mild mossaic | |
Line-5 | 3/5 | Mild mossaic | |
MYMIV AC2-AC4 | Line-6 | 5/5 | No Symptoms |
Line-15 | 4/5 | No Symptoms | |
Line-17 | 3/5 | Mild Mossaic | |
Untransformed Control | 0/5 | severe symptoms in all plants |