Table 4. Substrate loading phase of lagging strand model.

Reagent	[Final]	1x (μl)	3x (μl)
ddH2O	-	5.3	16.7
5x TDBG	1x	2.2	6.6
10x MK	1x	1.1	3.3
150 nM phiX174:Pr1b	1.5 nM	0.25	0.75
20 μM RPA	600 nM	0.8	2.3
3 mM dATP	60 μM	0.5	1.5
3 mM dGTP	60 μM	0.5	1.5
100 mM ATP	0.5 mM	0.13	0.4
	TOTAL:	11.0	33.0