Figure 5.

Chromosomal distribution of DMRs in HF compared with (a) control, hypermethylation clusters on Chrs 4 and 18 and qRT-PCR of these hotspot hypermethylated genes in (b) 26-week- and (c) 5-week-old liver. (a) There were 3360 DMRs assigned by Chr. Exposure to HF diet induced the most hypermethylation on Chr 4. Hypermethylation DMRs are indicated by black lollipops. One lollipop represents five hypermethylated DMRs. There were 211 DMRs found on Chr 4, with 120 DMRs located in the atherosclerosis QTL 1 (black underline). There were 84 DMRs found on Chr 18, with 58 DMRs located in the insulin-dependent diabetes susceptibility 21a QTL (black underline). mRNA expression in (b) 26-week and (c) 5-week liver (target normalized to 36B4 in arbitrary units; n = 4 to 5 per group). *P < 0.05 using an unpaired two-tailed Student t test.