Table 2.
qRT-PCR of Genes Involved in Metabolism, Cell Signaling/Development, and Transcription Regulation in 5-Week-Old Liver
Metabolism and Cell Signaling/Development | Control | HF | Pathway |
---|---|---|---|
Aldh1l2 | 1.38 ± 0.57 | 3.84 ± 0.51a | Amino acid metabolism |
Mapkapk2 | 1.09 ± 0.20 | 2.92 ± 0.73a | Carbohydrate metabolism |
Dusp4 | 1.15 ± 0.27 | 3.72 ± 0.78a | Cell to cell signaling |
Tp53 (Trp53) | 1.18 ± 0.40 | 2.38 ± 0.28a | Cellular development |
Ptprf | 1.02 ± 0.10 | 3.13 ± 0.89a | Lipid metabolism |
Timp3 | 1.25 ± 0.46 | 5.06 ± 1.13a | Lipid metabolism |
Trio | 1.16 ± 0.30 | 2.00 ± 0.18a | Lipid metabolism |
ILK pathway | Control | HF | Pathway |
Itgb4 | 1.15 ± 0.28 | 2.49 ± 0.48a | ILK pathway |
Mmp9 | 1.34 ± 0.45 | 2.92 ± 0.43a | ILK pathway |
Sh2b2 | 1.07 ± 0.18 | 1.65 ± 0.17a | ILK pathway |
Tgfb1l1 | 1.04 ± 0.14 | 2.52 ± 0.60a | ILK pathway |
Transcription regulator | Control | HF | Function |
Etv6 | 1.08 ± 0.21 | 2.59 ± 0.54a | Cell-to-cell signaling and interaction |
Tgfb1l1 | 1.04 ± 0.14 | 2.52 ± 0.60a | Cellular development, growth, and proliferation |
Jdp2 | 1.05 ± 0.18 | 4.19 ± 1.33a | Hereditary disorder |
Sox9 | 1.11 ± 0.25 | 6.34 ± 1.75a | Lipid metabolism |
Elf3 | 1.03 ± 0.13 | 2.21 ± 0.42a | Lipid metabolism, cell signaling |
Nfia | 1.27 ± 0.49 | 3.04 ± 0.36a | Cellular development |
Values are mean ± SEM or as otherwise indicated. Results are presented as target normalized to 36B4 in arbitrary units; n = 4 to 5 per group.
P < 0.05 using an unpaired two-tailed Student t test.