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. 2017 Jun 28;158(9):2944–2954. doi: 10.1210/en.2017-00145

Figure 4.

Figure 4.

Runx1 is a downstream target gene for androgen-mediated modulation of Ezh2 expression and activity. Runx1 mRNA levels in (a) mouse GCs (mGCs) and (b) KGN cell line treated with nonspecific (Nsp) and Ezh2-specific siRNA and stimulated with media alone, hCG for 4 hours, or DHT (25 nM) for 48 hours or DHT priming for 48 hours followed by hCG for 4 hours. (c) Confirmation of Ezh2 knockdown. Data are displayed as mean ± standard error of the mean (n = 3 experiments) and normalized to GAPDH levels. *P ≤ 0.05, **P ≤ 0.02. (d and e) Pattern of Runx1 mRNA levels in (d) mouse GCs and (e) KGN cell line pretreated with DHT (25 nM) for different durations followed by media or hCG stimulation (4 hours) in presence or absence of the PI3K inhibitor LY294002 (10 μM). Data are displayed as mean ± standard error of the mean (n = 3 experiments) and normalized to GAPDH levels. *, **, ***P ≤ 0.05, #P ≤ 0.005 (* vs corresponding time point of media, ** vs LY294002 plus hCG, *** vs 24-hour LY294002 plus hCG, and # vs LY294002 plus hCG and 24-hour hCG treatment).