Figure 5.

Androgen-induced phosphorylation of Ezh2 at serine 21 is necessary for Runx1 expression. Pattern of RUNX1 mRNA expression in EZH2 knockdown KGN cells overexpressing either wild-type or mutated EZH2 and stimulated with media alone or hCG for 4 hours, DHT (25 nM) for 48 hours/72 hours, or DHT priming for 48 hours/72 hours followed by hCG for 4 hours. Data are displayed as means ± standard error of the mean (n = 3 experiments) and normalized to GAPDH levels. *^, **P ≤ 0.05 (* vs media and ** vs hCG). Western blot results are representative of three separate experiments.