Figure 5.

Crisp4 mRNA expression is reduced in ob/ob mice. (a) Graph showing the percentage of copulatory plugs of WT (dashed line) and ob/ob (black line) mice when mated with WT females for 7 days (four female/male; n = 6 to 10 males). Log–rank (Mantel–Cox) test was performed. (b) Bar graph showing Crisp4 gene expression in the testis of WT and ob/ob mice. Unpaired two-tailed Student t test was performed. (c) Bar graph showing the Crisp4 expression in the epididymis of adult (P60) WT and ob/ob mice, and WT prepubertal (P21) mice. Kruskal–Wallis test of one-way ANOVA was performed. Data are expressed as mean ± standard error of the mean (n = 4 to 10). *P < 0.05; **P < 0.01; ****P < 0.0001. (d) Representative image of the testis of adult (P60) WT and ob/ob mice, and prepubertal (P21) WT mice. (e and f) Bar graphs showing (e) the percentage of motile sperm and (f) the percentage of two-cell embryos that developed into blastocysts after in vitro fertilization of WT eggs with sperm from WT, ob/ob, and ob/ob treated with 1.5 µM CRISP4 (ob/ob + CRISP4) mice. Kruskal–Wallis test of one-way ANOVA was performed. Data are expressed as mean ± standard error of the mean (n = 5 to 9 males). (a and b) Different letters = differences (P < 0.05).