Figure 1.

Changes in Sertoli cell number after treatment with different doses of DTX. (a) Neonatal (2 days) or (b) adult (50 days) iDTR mice were injected subcutaneously with different doses of DTX, and Sertoli cell numbers were measured when the animals were (a) 9 days old (7 days after injection) or (b) 80 days old (30 days after injection). Sertoli cell numbers for individual animals are shown. Differences between groups are shown by the letters above each group; groups that do not share a common letter are significantly different. Examples of hematoxylin and eosin−stained sections from different groups of animals treated with DTX (a) as neonates or (b) as adults are shown above each graph. The bars represent 100 µm.