Figure 6.
SWATH (MS/MSALL)-based lipid profiling of mouse serum. (A) PCA of chow and HFD with and without metformin (MetF). Most changed (B) positive ion and (C) negative ion lipids characteristic of MetF effects on HFD-fed mice. Bars indicate lipid differences with P ≤ 0.05, n = 3 mice for all groups, labeled as MA, MB, and MC (mouse A, B, and C, respectively). CE, cholesteryl ester; CL, cardiolipin; DAG, diacylglycerol; GM1, GM2, gangliosides M1 and M2; HexCer, monohexosylceramide; LPC, lysophosphatidylcholine; MAG, monoalkyldiacylglycerol; PCA, principal component analysis; PG, phosphatidylglycerol; PI, phosphatidylinositol; TAG, triacylglycerol.