Effect of long-term NVP-BGJ398 treatment expression of mineralization genes in HMWTg mice. Starting at 5 weeks of age, HMWTg mice were treated with the FGFR inhibitor NVP-BGJ398 (50 mg/kg, oral) or vehicle three times per week for 8 weeks. Vector mice were treated with vehicle only. Mice were euthanized 24 hours after the last NVP-BGJ398 administration. Whole femurs were collected and used for RNA extraction. Quantitative polymerase chain reaction analysis was performed for the following gene expression: (a) Fgf2, (b) FGFR3c, (c) FFGFR1c, (d) PTHR1, (e) Fgf23, (f) Mepe, (g) Opn, (h) Dmp1, (i) Bsp, and (j) Mgp. Data are mean ± SE, n = 10 to 15 per group. *Vector-Vehicle vs HMWTg-Vehicle, P < 0.05; #Vector-Vehicle vs HMWTg-NVP-BGJ398, P < 0.05; @HMWTg-Vehicle vs HMWTg-NVP-BGJ398, P < 0.05.